Prof. Dr. Roseline Nussbaumer
Institute iPrint, School of Engineering and Architecture Fribourg, member of the University of Applied Sciences and Arts Western Switzerland (HES-SO).
Multi-Nozzle Inkjet and its Potential for the Industrialization of Bioprinting
Drop-on-demand bioprinting, thanks to the unique flexibility and spatial accuracy it offers, has a promising future for the deposition of living cells and extracellular matrices towards the fabrication of physiologically relevant 3D tissue models. The creation of a functional vascular network remains a major technological challenge for the development of engineered tissues and organs. Efforts to construct large 3D tissues (?1 cm of thickness) have been hampered by the lack of transport system for nutrients and oxygen supply within the tissue core. In the meantime, bioprinting is currently mostly achieved by deposition through a single-nozzle system, which strongly limits the upscaling potential of this technology into industrialization.
To address these challenges, iPrint specifically investigates multi-nozzle inkjet printing as a high-throughput and high-resolution industrial 3D bioprinting technology. We study the reliability of the printing process, its throughput and its impact on cells by modifying the rheological properties of the bioink (cell-laden ink). The bioinks are tailored to satisfy both printability and cell function. By co-printing multiple inks (bioinks and a fugitive vascular ink), according to a predefined 3D pattern, we make the proof of concept that this technology allows for the engineering of perfusable thick vascularized tissues. Bioink composition, printing process and printing parameters have been determined for cells, hydrogels and fugitive ink deposition.
Prof. Roseline Nussbaumer, PhD, is Professor of Mathematics at the School of Engineering and Architecture of Fribourg (HEIA-FR), a member of the University of Applied Sciences and Arts Western Switzerland (HES-SO) since 2007. With a master in Mathematics and a minor in Physics at the University of Fribourg, she realized an interdisciplinary research (PhD) at the institute of Physiology, in collaboration with the institute of Mathematics of the University of Fribourg: modeling of muscle activation.
With expertise in mathematical modeling and knowledge in the field of neuroscience, she joined the iPrint Institute in 2013 and contributes to the development of the research area of ‘Printing in live science’ and ‘Bioprinting’.